The 2nd International Symposium on the Molecular Detection of Trophic Interactions will be held in Lexington, Kentucky, USA, from Monday 13 May 2013 – Friday 17 May 2013. The conference will focus on all aspects of trophic interactions in terrestrial, freshwater and marine ecosystems and build on information presented at the first international symposium, held in Innsbruck, Austria, in 2007.