Advanced Immunoenzyme Histochemistry with special emphasis on multiple staining techniques

This international short-course aims at training participants to perform multiple staining techniques in a hospital or research laboratory setting. All aspects for performing multiple immunoenzyme staining will be discussed during the theoretical and practical sessions, using human and animal tissues. Furthermore, state-of-the-art experts in the field of immunohistochemistry/in situ hybridization techniques will provide theoretical and practical expertise with a special reference to multiple staining.
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Additional information:
The application of single immunohistochemical staining techniques of tissue specimens is widely spread among many biological sciences. However, the visualization of multiple antigens in one tissue specimen using different enzyme chromogens is believed to be restricted to some 'happy few' with 'golden hands'. This belief is justified by the fact that most multiple staining techniques are published as 'applied papers', strongly tailored to a particular aim and sometimes including the preparation of special reagents. This approach therefore cannot be regarded as 'generally applicable' and 'reproducible'. In contrast, the above mentioned course pays attention to multiple immunoenzyme staining methods which can be performed with commercially available reagents. It offers a guide through the many different multiple staining methods and color combinations, which usually frightens off the starter in this field. General protocols will be discussed and performed which can be easily adapted to detailed working procedures for many different multiple staining applications. Furthermore, attention will be paid to the Nuance™ spectral imaging system. Nuance™ consists of a camera and user-friendly software for ‘unmixing’ of multiple staining samples based on the spectral characteristics of the used chromogens. After unmixing into individual images, percentage of co-localization plus several standard imaging procedures can be applied.

This course has been developed for biomedical specialists (pathologists, immunologists, histologists, cytologists) and other researchers and laboratory technicians who are interested or involved in immunohistochemical methods. All others who are interested in these techniques are also invited to participate. Both participants working with human or animal material are welcomed. The participants should have a basic knowledge on immunohistochemistry and/or immunocytochemistry. For the practicals no specific experience is required, but some practical histochemical experience is desirable. The maximum number of participants for this course is 14.

Course structure:
This intensive course consists of a series of practical sessions interspersed with lectures and tutorials. 40% of the time is devoted to lectures and 60% is spent on "hands-on" experiments, covering techniques that may be directly applied in research and biomedical diagnostic laboratories. All participants will receive a practical manual containing all used protocols.

Guest Lectures:

In situ double staining techniques in Multiple Sclerosis research.
Ing. M. van Meurs, Dept. Immunology, Erasmus MC Rotterdam, NL

Sensitive multiple-target fluorescence detection and signal amplification systems for in situ hybridisation and immunocytochemistry
Dr. E.J. Speel, Dept. Molecular Cell Biology, University Maastricht, NL.

Immunogold/silver staining
Drs. P. van der Plas, Aurion, Immunogold/silver products, Wageningen, NL.

We just need some pictures! – Immunohistochemistry in research projects
Dr. Heike Göbel, Institute for Pathology, University Hospital of Köln (Cologne), Germany

Introduction to spectral imaging
Roslyn Lloyd, MSc, Caliper LS / PerkinElmer, UK

Course staff:

Course leader:
Dr. C.M. van der Loos, Department of Pathology, Academical Medical Centre (AMC) Amsterdam

Course co-ordinator:
Dr. Martie C.M. Verschuren, Avans Hogeschool, Readership Separation Sciences, Breda, NL

For more information about the course fee and how to register please visit our website.

9 Apr - 12 Apr 2013
meeting website