RNA 3' ends: Mechanism and biological function in eukaryotic genomes

Appreciation of RNA 3' end formation as a key regulatory step in gene expression has increased dramatically in recent years. We will start with transcription by considering how the chromatin template is made ready for transcription initiation and how the end of the gene is connected to its beginning. This will lead into how processing of pre-mRNA is coupled to transcription elongation and how splicing is regulated via specific chromatin marks. More importantly transcription coupled 3' end processing of mRNA, small stable RNAs (snRNA, snoRNA), cryptic unstable RNAs in yeast (CUTs) and other non-coding RNAs in higher organisms will be discussed. Transcripts of well-over 50% of human genes are now known to undergo alternative polyadenylation (APA.). We will examine the underlying mechanisms, and the important role played by APA in cell differentiation and disease. Here the impact of genomic analysis is especially dominant since most transcripts display variable 3' ends, conferring different regulatory properties on mRNAs. Superimposed over productive transcription, many coding and non coding RNAs follow the same expression pathways but are somehow distinguished from functional RNA and subjected to rapid degradation. This selection process will also be extensively covered during the workshop.
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Keynote Lecture: James Darnell “RNA 3’ends”
Oral session 1 (3.00 pm – 5.30 pm):
Mechanisms of alternative polyadenylation

Shona Murphy “Role of variant U1 snRNAs in polyA site selection”
Raul Mendez “Nuclear role for CPEB in alternative polyadenylation”
Gideon Dreyfuss “U1 snRNA levels regulate polyA site selection”
Reception (6.00 pm -7.00 pm)
Dinner (7.00 pm -8.30 pm)


Oral session 2 (9.00 am-12.30 am):
Transcriptional initiation by RNA polymerase II; abortive and bidirectional initiation; early premature termination.

John Lis “Paused Pol II”
Karen Adelman “Abortive transcription initiation”
David Bentley “Premature termination”
Steve Buratowski “Interplay between ncRNA transcription and Pol II promoter selection”
Steve West “RNA turnover of abortive transcripts from 5’ ends”
1 short talk from selected participants
Lunch (1.00 pm – 2.00 pm)

Oral session 3 (2.30 pm - 6.15 pm):
Transcriptional elongation and coupling with RNA processing

Danny Reinberg “Chromatin and transcriptional elongation”
Dirk Eick “CTD code during transcription elongation”
Karla Neugebauer “Co-transcriptional splicing and chromatin structure”
Jean Beggs “Kinetics of splicing coupled to transcription”
Alberto Kornblihtt “Transcription elongation rates and alternative splicing”
2 short talks from selected participants
Dinner (7.00 pm -8.30 pm)
Poster Session 1st showing (8.30 pm - 10.00 pm)


Oral session 4 (9.00 am -12.30 am):
mRNA 3’ end formation; bioinformatic aspects.

Mihaela Zavolan “Cleavage polyA factors and alternative polyadenylation”
Jeffrey Corden “Nrd1, Nab3 and Sen1 mediated RNA 3’ end formation”
Bin Tian “Bioinformatics of polyA site selection”
Lars Steinmetz “Transcriptomes in yeast”
Monika Gullerova “PolyA signals in S. pombe” (short talk)
2 short talks from selected participants
Lunch (1.00 pm – 2.00 pm)

Oral Session 5 (2.30 pm-6.15 pm):
Mechanism of transcriptional termination: promoter terminator interactions

Maria Carmo-Fonseca “Chromatin signatures of gene looping”
Mike Hampsey “Shared promoter terminator factors”
Caroline Dean “Role of non coding RNAs in transcriptional termination in Arabidopsis”
Liang Tong “Structural biology of transcriptional termination”
Domenico Libri “Termination factors in yeast”
Sue Mei Tan-Wong “R-loops and bidirectional transcription” (short talk)
1 short talk from selected participants
Dinner (7.00 pm - 8.30 pm)
Poster Session 2nd showing (8.30 pm - 10.00 pm)


Oral session 6 (9.00 am-12.30 am):
RNA 3’end formation and non coding RNAs

Torben Jensen “Promoter and terminator associated non coding RNAs”
Jane Mellor “Antisense terminator transcripts”
Francoise Stutz “Role of antisense transcription in gene silencing in S. cerevisiae”
Irena Bozzoni “Termination of transcription of primary microRNAs
Francois Bachand “Polyadenylation of lncRNA”
1 short talk from selected participants
Lunch (1.00 pm – 2.00 pm)

Oral Session 7 (2.30 pm-6.15 pm):
Interconnections between RNA 3’ end formation and other aspects of regulated gene expression

Joan Steitz “RNA 3’ end formation in complex viruses”
Joel Richter “Polyadenlation in neuronal development”
Bill Marzluff “Histone mRNA 3’end formation and the cell cycle”
Tom Blumenthal “3’ end formation in polycisronic transcripts of C. elegans”
Jim Manley “RNA 3’ end formation; interconnections with other regulatory pathways”
2 short talks from selected participants
Reception (6.30 pm -7.30 pm)
Gala dinner (7.30 pm – 9.00 pm)
Entertainment (9.00 pm onwards)

25 Sep - 29 Sep 2013
United Kingdom
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