Cryo-Electron Microscopy and 3D Image

Why You Should Apply

The course provides a thorough theoretical and practical background in a wide variety of aspects of molecular cryo-electron microscopy including sample preparation and data collection as well as the many different aspects of image processing. This wide variety of aspects fosters the integration of knowledge and methods, which is the key for achieving top results in molecular electron microscopy. Practical sessions during the course are focussed on the major topics in image processing for cryo-electron microscopy including subtomogram averaging, single particle processing, 2D-crystal processing, and reconstruction of helical particles.

Aims of event
The course is aimed at PhD-students and PostDocs, who have already some experience in electron microscopy and want to use electron cryo microscopy and learn advanced image processing techniques. We ask that the students have the possibility to use at least one of the techniques taught in the course in their projects. However, there is no prior knowledge of cryo techniques and/or image processing required.


Lectures - to provide general background and in depth knowledge required for electron cryo microscopy and image processing.

Practicals – Each student will carry out one main practical during the course. The main practicals cover the most common fields of 3-D image analysis 1) cryo-tomography with subtomogram averaging, 2) single particle processing, 3) 2D-crystal processing, and 4) reconstruction of symmetrical particles (focusing on helical reconstruction together with details of high-resolution resolution structure solving). Students will also do a second shorter practical. Here the students have a choice of 1) electron cryo microscopy (sample preparation, sample transfer, data acquisition) 2) an introduction to one of the other image processing techniques or 3) working on their own projects, getting detailed supervision and input from the teachers.

+ show speakers and program

Martin Beck, EMBL Heidelberg, Germany

Bettina Böttcher, University of Edinburgh, United Kingdom

John Briggs, EMBL Heidelberg, Germany

Sarah Butcher, University of Helsinki, Finland

Friedrich Foerster, MPI Biochemistry, Germany

Achilleas Frangakis, University of Frankfurt, Germany

Richard Henderson, MRC Laboratory of Molecular Biology, United Kingdom

Juha Huiskonen, University of Oxford, United Kingdom

Ron Milligan, Scripps Research Institute, USA

Deryck Mills, MPI Biophysics, Germany

Ardan Patwardhan, Electron Microscopy Data Bank, EBI, United Kingdom

Pawel Penczek, University of Texas, USA

Juergen Plitzko, MPI Biochemistry, Germany

Carsten Sachse, EMBL Heidelberg, Germany

Carlos Oscar Sánchez Sorzano, National Center of Biotechnology (CSIC), Spain

Christiane Schaffitzel, EMBL Grenoble, France

Michael Schatz, Image Science Berlin, Germany

Sjors Scheres, MRC Laboratory of Molecular Biology, United Kingdom

Rasmus Schroeder, University of Heidelberg, Germany

Henning Stahlberg, University Basel, Switzerland

Vinzenz Unger, Northwestern University, USA

26 Aug - 2 Sep 2012
meeting website