Fundamentals of Modern Methods of Biocrystallography - BioCrys2012

The sixth BioCrys course will be focused on Macromolecular Crystallography, by developing the fundamental concepts of Crystallography and illustrate these by tutorial and practical sessions.

This is particularly important due to the rapid development of the field and also due to the fact that many young researchers do not receive a formal education in Crystallography. Additionally, with the introduction of high-throughput methods, the need to understand and grasp the theory behind modern methods will certainly be critical to overcome the difficult problems that will arise and will not be solved by the automated procedures.

Topics of the course will run from fundamentals such as symmetry, point groups and crystal systems, basic diffraction physics, reciprocal space and the Ewalds sphere, radiation damage, data processing, symmetry in the diffraction pattern, structure factors, Patterson function to modern methodologies including molecular replacement, SAD, MAD, MIR and maximum likelihood phasing, direct methods, density modification, refinement, model building, twinning and structure validation. Main challenges on sample preparation and crystallization of proteins will also be covered.

The course will be organized with lectures in the mornings and interactive practicals and tutorials in the afternoons. Evening lectures will address two main important topics within Structural Biology, one covering studies of membrane proteins and the other the beginning of use of free electron lasers in macromolecular Crystallography.

Participants will be limited to 36, aimed primarly at people at the beginning of their crystallographic research activity as PhD students or others. Selected applicants are invited to present a poster during the course.
+ show speakers and program
Margarida Archer ITQB, Oeiras, PT
Isabel Bento ITQB, Oeiras, PT
Gabor Bunkoczi University of Cambridge, UK
Kevin Cowtan University of York, UK
Zbigniew Dauter Argonne National Laboratory, USA
Carlos Frazão ITQB, Oeiras, PT
Elspeth Garman University of Oxford, UK
Christoph Hermes EMBL c/o DESY, Hamburg, DE
Edward Hough University of Tromso, NL
Gordon Leonard ESRF, Grenoble, FR
Andrew Leslie MRC LMB, Cambridge, UK
Bernhard Lohkamp Glasgow University, UK
Adrian Mancuso European XFEL GmbH, Hamburg, DE
Pedro Matias ITQB, Oeiras, PT
Rob Meijers EMBL-Hamburg, DE
Poul Nissen University of Aarhus DK
Anastassis Perrakis NKI, Amsterdam, NL
Célia Romão ITQB, Oeiras, PT
Thomas Schneider EMBL-Hamburg, DE
Clemens Vonrhein Global Phasing Ltd, Cambridge, UK

20 Oct - 27 Oct 2012
meeting website